MP08-04 PHENOTYPIC CHARACTERIZATION OF A Mas1 RECEPTOR WILDTYPE AND KNOCKOUT ON BLADDER PAIN IN RATS

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology Pharmacology (MP08)1 Sep 2021MP08-04 PHENOTYPIC CHARACTERIZATION OF A Mas1 RECEPTOR WILDTYPE AND KNOCKOUT ON BLADDER PAIN IN RATS Maia Terashvili, Bidyut Medda, Bhavana Talluri, Banani Banerjee, and Jyoti Sengupta TerashviliMaia Terashvili More articles by this author , MeddaBidyut Medda TalluriBhavana Talluri BanerjeeBanani Banerjee SenguptaJyoti View All Author Informationhttps://doi.org/10.1097/JU.0000000000001981.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION OBJECTIVE: The Angiotensin is widely known for its roles in blood pressure fluid homeostasis. Recently it has been implicated several facets metastatic bone disease including inflammation, angiogenesis, tumor cell proliferation, migration. In addition, a model prostaglandin-induced hyperalgesia, Ang-(1-7) dose-dependently attenuated behavioral signs pain. Aim: study was undertaken test the hypothesis that Mas receptor activation specific agonist could be an effective therapeutic target alleviate bladder pain syndrome and/or chronic pelvic METHODS: Two groups female rats: wild type (Mas R WT) knockout (KO) were used study. electromyographic (EMG) recording from external oblique muscles (EOM) abdomen during urinary distension (UBD) direct method measuring sensation awake rats. these experiments, rats anesthetized with 2% Isoflurane pair Teflon-coated electrodes chronically implanted into EOM EMG recordings. Three days after surgery, before viscero-motor reflex (VMR) UBD lightly isoflurane polyethylene catheter (PE-50) inserted transurethrally sealed tissue glue prevent voiding. This allowed us generate intravesical without spontaneous voiding, obstruction distended saline either slow infusion (0.1ml/min) or graded phasic isobaric distensions (10, 20, 30, 40 60mmHg). increasing contractions incrementing pressures considered as cramping represented VMR. RESULTS: WT exhibited threshold VMR at distending >20mmHg, whereas KO exhibits <10mmHg higher. results suggest are more sensitive visceral compared (FDistension (5, 66) = 132.3, p <0.0001). injected AVE0991, agonist, abolished WT, but not CONCLUSIONS: Based on data may play role perception. Ang (1-7)/Mas axis Source Funding: work supported NIH 2 R01 DK099201-05 partly Digestive Disease Center funds J.N. © 2021 American Urological Association Education Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e151-e151 Advertisement Copyright Permissions© Inc.MetricsAuthor Information Expand Loading ...